Unpack the microarrayer and check all items according to the packaging list.
Please visually inspect all items for damage may occur during transportation. Report damage to LabNEXT ( support at labnext.com ) and to the carrier. Do not operate damaged equipment.
Install the system on the lab bench or table. The place should be equipped with 100-240 VAC power jack and vacuum source either form central vacuum tube or portable vacuum pump.
- Remove plastic wrap from the cabinet.
-Open the cover and replace the door handle from the inner to the outer surface of the cabinet door. (The handle attached to the inner surface of the door intentionally, to prevent glass from damage during transportation).
- Remove protective foam tubes from the rails of the microarrayer.
Computer and Electric Connection.
- Install the control software on the PC that will be working with the system. Please refer to the Software Installation section of this manual.
- Connect the microarrayer power supply connector to the recepticle on the back side of the microarrayer.
- Plug the cord of the power supply in the power
- Connect the USB cable included in the package to
the USB port of the PC and to the USB connector of the microarrayer.
Do not connect or disconnect power supply connector +48VDC from the microarrayer when the system is in operation. If this happens you may need to restart the microarrayer controller or restart the PC.
There is no ON/OFF switch on the microarrayer or
its power supply. The system can be stopped from the PC by pressing <Ctrl-C>
combination of the keys at any time. It is also possible to disconnect the power
supply from the 110 - 240 VAC power jack when the system is running.
Run "ManualControl" utility by clicking at the icon
In the “LabNEXT Manual Control” dialog click on the “H” button in the center.
The microarrayer should start moving towards far left corner of the work board and to the upper position by the Z (vertical) axis. It comes to stop after finding the “Home” position.
Clicking on the buttons with arrows >> allows moving the microarrayer manually on the directions showed on the buttons. One arrow > indicates slower movements – one step per click. Two arrows – faster movements 100 steps per click.
If the microarrayer moves in all directions
controlled manually and finds “Home” position it connected correctly.
Launching Pre-defined Print Routines
Launch one of the pre-programmed print routines to check that calibration of the system have not changed during transportation.
Launch the “JobControl” utility by clicking on the icon
In the “Choose LabNEXT Print Job” dialog press the
<Load Existing Job> button to select print job “slide_1_spot_on_each_slide” if
you are printing on glass slides or “plate_1_spot_in_corner_wells” if you are
printing in 96-well microplates from the list of the pre-programmed print jobs.
NOTE: Do not insert microarray pins into
the printing head of the spotter for the first run. If the calibration was lost
during transportation the microarrayer can damage the pins.
Run selected print job following prompts of the
software. Observe microarrayer movements till the system stops and the Command
Prompt window (black window) disappears from the screen.
Put 384 well plate in the source plate nest
Put substrates (either slides or microplates) into the substrate holders of the system
Insert one pin in the left closer hole of the printing head;
Run the same print job again.
Observe the arrayer performs two washing and drying cycles in the beginning of the print routine. Check if the pin correctly access the holes of the washing and drying station. The pin should go inside the washing reservoir through the center of the hole without touching the walls. In the hole of the dryer the pin should go till its conical part is fully inserted in the smaller inner hole.
After performing washing/drying cycle the microarrayer should perform pickup from the source plate and deposition of the samples on all substrates. In case of the glass slide configuration the microarrayer deposits one spot in the center each substrate. In case of microplate configuration running the print job “plate_1_spot_in_corner_wells” the microarrayer should deposit samples in the centers of four corner wells of two 96-well microplates.
If the microarrayer correctly access all points it's ready for operation. You can use predefined printing routines or design your own using the “JobWizard” utility.
If it misses washing station or access labware with significant errors it requires calibration which can be done using “ConfigureSystem” utility. Please refer to the System Calibration section of this manual or contact technical support (support at labnext.com) for assistance.