μArrayer User Manual

μArrayer Parts and Controls

uArrayer parts and controls chart
1. Print handle. Rotate the Print Handle for continuously printing a row of microarray spots. The spots are printed with the intervals specific to the model of the uArrayer. u700 deposits spots 700um apart from each other. With each revolution the arrayer moves print head 0.7 mm, lowers the print head to produce a spot and lifts the print head. With clockwise rotation the spots are printed from left to right. With counter-clockwise rotation the spots are printed from right to left. 2. Slide Shift Dial. Use the Slide Shift Dial for shifting slide holder with the the slide to start a new row of spots. One revolution of the dial moves slide 700um. The slide can be moved to distances smaller or bigger than 700um by turning the dial to less or more than full revolution. 1/2 revolution results in 350um shift. 2 revolutions result in 1400 um shift. 3. Free Movement Dial. Use the Free Movement dial to move the head left or right, without making spots. The dial will rotate only if the pin is lifted over the slide surface. 4. Slide Holder. Accommodates one substrate 75mm x 25mm (3" x 1") 5. Xtend Pin Hole - 2.4mm. Hole in the print head for LabNEXT Xtend and Xtend RM microarray pins. 6. ChipMaker Pin Hole 1/16". Hole in the print head for ChipMaker series pin, manufactured by TeleChem, Inc. 7. Stealth Pin Hole - 1/16". Hole in the print head for Stealth series pin, manufactured by TeleChem, Inc.

Pin Cleaning and Sample Pickup

Sample pickup and pin cleaning between the samples has to be done manually. For pin cleaning the pin has to be taken out of the print head. Dip the pin in a reservoir with washing liquid and then dry it in the Vacuum Dryer, provided with the uArrayer. Sample pickup is done by dipping the pin it in a reservoir with a sample. After pickup place the pin in the print head and continue printing another series of spots. uArrayer with pin dryer
Vacuum Dryer next to uArrayer. NOTE: Xtend pins (due to tubular construction ) can be preloaded with a fairly large amount of sample. Each pin can hold its own sample. In this case pins can be replaced in the print head after certain number of spots. No pin cleaning is required, since each pin holds only one sample.

Vacuum Dryer

Vacuum Dryer is designed for manually drying capillary and split pins after washing them in a washing solution. The Vacuum Nozzle is connected to a vacuum source (vacuum pump or a central vacuum pipe) and provides a steady flow of air through the nozzle in the middle of the body. The airflow will go through the pin placed in the nozzle. pin dryer parts
1. Vacuum Nozzle. Privides airflow for drying microarray pins. 2. Vacuum tube fitting. Connects vacuum nozzle to a vacuum source. 3. Suction cup. Firmly attaches the vacuum nozzle to flat surfaces. 4. Print Pin. Microarray pin inserted in the vacuum nozzle.